APAF1 anticorps (AA 1-464)

N° du produit ABIN5690621

Cet anticorps anti-APAF1 est un anticorps Rat Monoclonal détectant APAF1 dans WB, IHC, ELISA, IP et ICC. Adapté pour Humain.

Aperçu rapide pour APAF1 anticorps (AA 1-464) (ABIN5690621)

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp APAF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 2E12

Détail du produit anti-APAF1 anticorps

Épitope: AA 1-464

Specificité: Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.

Pureté: > 95% (SDS-PAGE)

Immunogène: Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Immunohistochemistry: (paraffin sections, 10 μg/mL) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al., (2000)) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for at least 1 year after receipt when stored at -20°C.

Détails sur APAF1

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: Apaf-1

Sujet: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

ID gène: 317

UniProt: O14727

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity